Back
For best output, select "Paper Size" as "A4" and "Margin" as "0" or "None".
To save or print to PDF, please select Print Destination > Save as PDF, enable Background Graphics under "More Settings", then click "Save".
During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Podocyte foot process (FP) morphology is a pivotal determinant of glomerular filtration barrier integrity. Owing to their nanoscale dimensions, FPs have traditionally been analyzed by electron microscopy (EM), a labor-intensive and low-throughput technique that limits large-scale or comparative studies. To overcome these constraints, we developed the Podocyte Exact Morphology Measurement Procedure (PEMP)—a super-resolution microscopy (structured illumination microscopy, SIM)-based method enabling precise, reproducible, and largely automated quantification of FP morphology, particularly filtration slit density (FSD), in standard paraffin-embedded kidney tissue.
PEMP combines multichannel immunofluorescence labeling (e.g., nephrin/podocin, synaptopodin/integrin α3) with SIM imaging to capture nanoscale structural detail. A proprietary software and AI-assisted analysis pipeline developed at NIPOKA performs fully automated FSD quantification, minimizing observer bias and ensuring consistent cross-sample comparisons. Except for immunofluorescence staining and slide loading, the workflow is fully automated, making PEMP readily applicable to both research and diagnostic laboratories
We validated PEMP across diverse settings. In nephrotoxic serum (NTS) injury models, PEMP detected a significant reduction in FSD within 24 hours post-injury—preceding detectable proteinuria. In a translational feasibility study of over 135 human kidney biopsies encompassing various glomerular diseases, FSD values correlated with clinical and functional parameters, offering enhanced morphometric insights compared to EM. Furthermore, in a murine aging cohort, PEMP revealed a progressive, age-dependent decline in FSD and increased glomerular diameter, highlighting its sensitivity to both pathological and physiological remodeling.
Together, these findings establish PEMP as a robust, scalable, and reproducible platform for quantitative analysis of podocyte architecture in preclinical and clinical contexts. Its compatibility with routine histology, high throughput, and minimal user input position PEMP as a next-generation tool for glomerular pathology, with significant potential for early disease detection, longitudinal monitoring, and aging research in translational nephrology.
