EVALUATION OF CORTICAL MICROCIRCULATION IN KIDNEY TRANSPLANTS USING LINEAR TRANSDUCER DOPPLER ULTRASOUND: DELAYED VS. IMMEDIATE GRAFT FUNCTION

 

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https://storage.unitedwebnetwork.com/files/1099/0b8798ce8887d3f5be54f0a06e3916eb.pdf
EVALUATION OF CORTICAL MICROCIRCULATION IN KIDNEY TRANSPLANTS USING LINEAR TRANSDUCER DOPPLER ULTRASOUND: DELAYED VS. IMMEDIATE GRAFT FUNCTION

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Karmen
Romozi
Karmen Romozi karmen.romozi@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia *
Miha Arnol miha.arnol@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia -
Jernej Pajek jernej.pajek@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia -
Jakob Gubenšek jakob.gubensek@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia -
Andreja Aleš-Rigler andreja.ales@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia -
Jadranka Buturović-Ponikvar jadranka.buturovic@kclj.si University Medical Centre Ljubljana Department of Nephrology Ljubljana Slovenia -
 
 
 
 
 
 
 
 
 

Due to its non-invasiveness and accessibility, Doppler ultrasound (US) is the first-line imaging modality for morphological assessment and evaluation of hemodynamic parameters in transplanted kidneys. Typically, curved transducers are used; however, because transplanted kidneys are located close to the skin surface, they are uniquely accessible for Doppler examination using a linear transducer. This allows for more precise evaluation of cortical blood flow parameters. The early postoperative period following kidney transplantation is characterized by significant macrovascular and microvascular changes in the graft. This prospective observational study aimed to compare daily Doppler US parameters of kidney grafts between recipients with immediate graft function (IGF) and those with delayed graft function (DGF).

Consecutive patients who received a kidney transplant between August 2015 and September 2018 were enrolled in this prospective observational study. Patients with primary non-function, early graft loss, or early post-transplant death were excluded. In addition to daily laboratory testing, Doppler ultrasound examinations were performed using a linear transducer on weekdays during the first 14 postoperative days or until discharge, and again at one month post-transplantation, targeting the region of the graft closest to the skin surface. Examinations were conducted by nephrologists with expertise in renal ultrasonography using a Siemens ACUSON Sequoia ultrasound system in the majority of cases. Serum creatinine (sCr), cortical perfusion (CP) (see Image 1), resistive index (RI), and end-diastolic velocity (EDV) (see Image 2) were compared between the IGF and DGF groups.

A total of 123 patients were included, of whom 84 (68%) were male, with a median age of 53 years (range: 18–81). Most patients (119; 97%) received a kidney from a deceased donor, while 4 (3%) received a graft from a living-related donor. Ninety-nine patients (80%) exhibited immediate graft function, while 24 (20%) developed DGF. Doppler US parameters and serum creatinine levels for the IGF and DGF groups are presented in Table 1 and Figures 1–3. Across all time points during the observation period, patients with DGF consistently showed poorer CP, higher RI, lower EDV, and worse graft function compared to those with IGF. However, these differences did not reach statistical significance, likely due to the limited sample size and technical challenges in performing early postoperative Doppler assessments with a linear probe (e.g., due to bowel gas or edema). Notably, the decline in serum creatinine preceded the improvement in Doppler parameters. By the end of the 30-day observation period, all patients were dialysis-independent.

Within the first 30 days post-transplantation, patients with delayed graft function exhibited persistently higher RI, lower EDV, and reduced cortical perfusion compared to those with immediate graft function. However, improvement in Doppler indices did not precede the recovery of kidney function in either group.

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