IMPROVING RENAL BIOPSY CORE ADEQUACY USING A BEDSIDE 20X PORTABLE MICROSCOPE WITH SMARTPHONE ATTACHMENT

 

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IMPROVING RENAL BIOPSY CORE ADEQUACY USING A BEDSIDE 20X PORTABLE MICROSCOPE WITH SMARTPHONE ATTACHMENT

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Amit Kumar
Mohanty
Amit Kumar Mohanty write4amit@gmail.com Asian Institute of Nephrology and Urology Hyderabad India Nephrology Hyderabad India *
Srikanth Gundlapalli srikanthnephro@gmail.com Asian Institute of Nephrology and Urology Hyderabad India Nephrology Hyderabad India -
Sujeeth Reddy Bande sujeethbande@gmail.com Asian Institute of Nephrology and Urology Hyderabad India Nephrology Hyderabad India -
Venkateswar Rao Mulpuru mulpuru1962@gmail.com Asian Institute of Nephrology and Urology Hyderabad India Nephrology Hyderabad India -
 
 
 
 
 
 
 
 
 
 
 

Adequate renal biopsy cores are essential for accurate histopathological diagnosis. Factors such as needle gauge and real-time glomerular assessment influence adequacy. This study evaluated whether the use of a 20x portable microscope with smartphone attachment enhances core adequacy and diagnostic yield in native and allograft kidney biopsies. Secondary objectives included comparing adequacy between 16G and 18G needles, assessing glomerular count estimation accuracy, and evaluating the correlation between core length and glomerular number.

This prospective-retrospective observational study was conducted over three years at a tertiary nephrology center and included 611 adult patients undergoing native or allograft renal biopsy. A total of 1133 cores were analyzed. Patients were divided into four groups based on needle size (16G or 18G) and the use of a 20x CARSON portable microscope with smartphone attachment. Four groups were compared: 18G without microscope (Group A), 18G with microscope (Group B), 16G without microscope (Group C), and 16G with microscope (Group D). Core adequacy, glomerular visualization, correlation of glomeruli number with histopathology and core length were studied. Bedside core length and glomerular estimates were recorded, and final values from biopsy reports were compared.

Comparison of Baseline Characteristics between four groups was done.

Comparison of Baseline Characteristics between four groups

The use of the 20x microscope significantly improved core adequacy from 74.56% to 90.37% with 18G (p<0.0001)and from 89.66% to 94.67% with 16G (p=0.086). IF diagnostic yield with 18G improved from 93.44% to 99.28% (p=0.0085). 

Core Adequacy Comparison with and without microscope in both 18G and 16G groups

Cores with no diagnostic yield decreased from 7.04% to 1.66% with 18G (p=0.0009) and from 2.59% to 0.94% with 16G (p=0.1953). 

Rate of Non-Diagnostic Biopsy Cores by Intervention Group

Mean glomerular count was significantly higher with 16G (13.36 ± 3.81) than 18G (8.43 ± 3.96), p<0.00001). 

Glomerular count estimation correlated strongly with final histology (r=0.83 for 18G, r=0.87 for 16G). 

Estimated VS Final Glomeruli Count - 18G

Core length showed poor correlation with glomerular number (r=0.30 for 18G, 0.19 for 16G).

The 20x portable microscope significantly enhances renal biopsy adequacy, particularly with 18G needles, and improves diagnostic yield by enabling real-time glomerular assessment. Reliance on core length alone is misleading, whereas glomerular visualization under portable microscope provides a reliable and practical tool to guide adequacy. Its most notable benefit is the ability to raise the adequacy of biopsies performed with the smaller 18G needle to a level comparable with those obtained using the larger 16G needle, effectively bridging the long-standing gap between procedural safety and diagnostic accuracy.

The content presented in this abstract was submitted for APCN x TSN 2025. Re-submitting the abstract is permitted by the organizers of the original meeting and written NOC certificate was obtained.

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