ASSESSMENT OF ANTI-BIOFILM EFFECTS OF SILVER-COATED PERITONEAL DIALYSIS CATHETERS AND CUFFS BY LOW-VACUUM SCANNING ELECTRON MICROSCOPY

 

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https://storage.unitedwebnetwork.com/files/1099/a23106becc9f0fa27855a4dc72a74230.pdf
ASSESSMENT OF ANTI-BIOFILM EFFECTS OF SILVER-COATED PERITONEAL DIALYSIS CATHETERS AND CUFFS BY LOW-VACUUM SCANNING ELECTRON MICROSCOPY

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Takefumi
Mori
Takefumi Mori tmori@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan * Tohoku Medical and Pharmaceutical University Division of Integrative Renal Replacement Therapy Sendai Japan
Takuo Hirose hirose.takuo@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan - Tohoku Medical and Pharmaceutical University Division of Integrative Renal Replacement Therapy Sendai Japan
Tsubasa Yuki yuki.tsubasa@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Maya Hariu saikin@hosp.tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Hospital Department of Clinical Laboratory Sendai Japan -
Kazuki Takano saikin@hosp.tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Hospital Department of Clinical Laboratory Sendai Japan -
Hiroki Ito hito@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Shigemitsu Sato sshige@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Integrative Renal Replacement Therapy Sendai Japan -
Chika Takahashi c.takahashi@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Integrative Renal Replacement Therapy Sendai Japan -
Kentaro Yano k.yano@hosp.tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Takahito Kaburagi kaburaki1996@hosp.tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Risa Ishikawa sr4111@hosp.tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Akari Endo endoakari820@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Ayaka Kamada ayaka.kamada@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Ikuko Oba-Yabana iku-yabana@tohoku-mpu.ac.jp Tohoku Medical and Pharmaceutical University Division of Nephrology and Hypertension Sendai Japan -
Yukari Kikuchi kikuyuka1147@gmail.com Tohoku Medical and Pharmaceutical University Division of Integrative Renal Replacement Therapy Sendai Japan -

Exit-site and tunnel infections are recognized as major complications of peritoneal dialysis (PD) and carry a risk of progression to severe peritonitis. Elderly patients with reduced activities of daily living (ADL) are particularly susceptible to infection due to multiple factors, including impaired immunity, delayed wound healing, cognitive decline leading to inadequate hygiene, and difficulty maintaining frequent bathing. The PD catheter is designed to prevent bacterial invasion into the peritoneal cavity by creating a subcutaneous tunnel and placing a cuff beneath the skin. The Dacron fiber cuffs not only secure the catheter subcutaneously but also act as a barrier against infection spread into the peritoneal cavity, as the catheter body itself does not adhere directly to the surrounding connective tissue. In this study, we evaluated the suppressive effects of silver coating on bacterial biofilm formation using both catheter and cuff components.

Staphylococcus aureus strains isolated from PD patients were used in the experiments. Conventional catheters and cuffs, as well as silver-coated catheters and cuffs, were incubated with the bacterial cultures. After fixation with ethanol and staining with phosphotungstic acid, bacterial adhesion and biofilm formation were observed using low-vacuum scanning electron microscopy (LV-SEM).

On conventional catheters and cuffs, Staphylococcus aureus exhibited clear biofilm formation. In contrast, although bacterial adhesion was observed on the silver-coated catheters and cuffs, the morphology of the attached bacteria differed markedly, and biofilm formation was suppressed. These findings suggest that silver coating alters bacterial adherence patterns and inhibits biofilm maturation on PD catheter surfaces.

Silver-coated PD catheters and cuffs demonstrated a potential suppressive effect against bacterial biofilm formation, indicating their possible utility in preventing PD-related infections. Further clinical and experimental studies are required to elucidate which structural factors—such as catheter and cuff design, the ratio and distribution of silver fibers, and the long-term durability of the coating—contribute most effectively to infection prevention. Further understanding of these mechanisms may lead to the development of safer and more infection-resistant PD devices, ultimately improving outcomes and quality of life in vulnerable PD populations, especially elderly or low-ADL patients.

Kewords