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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Protein-bound uremic toxins (PBUTs) such as p-cresyl sulfate (p-CS) remain a major limitation in hemodialysis due to their strong affinity for human serum albumin (HSA), which restricts toxin removal. Beyond this, p-CS can alter protein–membrane interactions, contributing to fouling and reduced membrane performance. Despite its clinical importance, the underlying mechanisms governing p-CS–induced adsorption behavior within hemodialysis membranes remain poorly understood. This work provides new insight into these interactions through high-resolution, in situ imaging and quantitative analysis of protein distribution across membrane layers.
High-resolution in situ Synchrotron Radiation Micro-Computed Tomography (SR-µCT) was performed at the BioMedical Imaging and Therapy (BMIT) beamline of the Canadian Light Source (CLS) to investigate the depth-dependent adsorption of HSA (50 mg/mL) on polyethersulfone (PES) membranes in the presence and absence of p-cresyl sulfate (p-CS, 20.1 mg/L). Image reconstruction and quantitative analysis were conducted using UFO and ImageJ software, enabling precise mapping of protein accumulation across individual membrane layers.
The presence of p-CS significantly increased HSA adsorption compared to control samples (p < 0.01), producing a sharp localized peak near layer index 30 with a maximum area of approximately 1.4 x 106 µ2. Adsorption declined rapidly beyond mid-membrane layers. These findings indicate that p-CS strengthens HSA’s affinity for PES membranes, likely through combined hydrophobic and electrostatic interactions, leading to more concentrated protein accumulation.
p-Cresyl sulfate significantly enhances HSA adsorption on PES membranes, leading to localized protein accumulation, membrane fouling, albumin loss, and reduced dialysis performance. These findings underscore the impact of PBUTs on altering membrane–protein interaction dynamics and support the design of next-generation hemodialysis membranes aimed at minimizing toxin-induced adsorption and improving overall treatment efficiency.
