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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Mesangial cells in a diabetic kidney play a major role in the initiation and progression of renal failure. Our previous studies have shown that the mesangial cell proliferation and matrix production are influenced by renal generation of nitric oxide. Most of these observations were made in cultured mesangial cells but how these effects are impacted by the ineraction with other renal cells is unknown. We tested the hypothesis that mesangial cell growth and proliferation as well as nitric oxide generation are altered in the presence of renal tubular cells.
To test our hypothesis, we co-cultured rat mesangial cells (RMC) and murine proximal tubular epithelial cells (TKPTS) in DMEM medium in six groups, each cell type alone and together, in low glucose (5.5mM) and high glucose media (25mM). The cells were grown for 48 hours and then detached from the flasks and cell proliferation was counted using improved Neubauer counting chamber. The media from all the flasks was collected to assess local cellular generation of nitric oxide in different cell groups. The NO generation was assessed by measuring NOx (total of nitrite and nitrate metabolites) using the Greiss reagent colorimetric assay.
The cell proliferation rate of RMC was higher in HG media than in LG while TKPTS grew slower in HG media., However, growing the cells together resulted in one cell suppressing the rate of proliferation of the other depending on the glucose levels. In LG, TKPTS cells outgrew RMC, the latter appearing sparse. However, with HG conditions, RMC grew faster but as TKPTS started populating the RMC became squeezed and fibrous looking. The NOx also showed distinct impact of co-culturing the two cell types. While in LG conditions, there was not much difference between cells grown as single types or in co-culture, under HG conditions, co-cultured cells produced higher NOx than either cell types under same conditions.
Co-culturing RMC and TKPTS impacted the cell proliferation rates of each other and enhanced the total Nox under HG conditions.
