BEYOND CATHETERS: A MULTISOURCE MICROBIAL AND RESISTANCE LANDSCAPE AMONG DIALYSIS PATIENTS

 

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BEYOND CATHETERS: A MULTISOURCE MICROBIAL AND RESISTANCE LANDSCAPE AMONG DIALYSIS PATIENTS

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Muh Arya
Prahmana
Lukman Pura lukman23003@mail.unpad.ac.id Universitas Padjadjaran Doctoral Study Program of Medical Sciences, Faculty of Medicine Bandung Indonesia - Dr. H. Abdul Moeloek Regional General Hospital Uro-Nephrology Department Bandar Lampung Indonesia
Ade Yonata adeyumi@gmail.com Dr. H. Abdul Moeloek Regional General Hospital Internal Medicine Department Bandar Lampung Indonesia - Universitas Lampung Faculty of Medicine Bandar Lampung Indonesia
Lina Herlina linakustaman@gmail.com Dr. H. Abdul Moeloek Regional General Hospital Clinical Microbiology Laboratory Bandar Lampung Indonesia -
Muh Arya Prahmana muharyaprahmana@gmail.com Universitas Padjadjaran Master Study Program of Biomedical Sciences, Faculty of Medicine Bandung Indonesia * Respiratory Programmatic Implementation and Research Institute Clinical Operations and Activities Department Jakarta Indonesia
Meti Metiani drmetimetiani@gmail.com Hermina Arcamanik Hospital Internal Medicine Department Bandung Indonesia -
Muhammad Syamsu Ramdhan syamsu.dr@gmail.com Dr. H. Abdul Moeloek Regional General Hospital Uro-Nephrology Department Bandar Lampung Indonesia -
Hasani Farhan hasanifarhan.md@gmail.com Dr. H. Abdul Moeloek Regional General Hospital Uro-Nephrology Department Bandar Lampung Indonesia -
Alvin Fauzie alvinauliaaf@gmail.com Oto Iskandar Dinata General Hospital Emergency Department Bandung Indonesia -
Raeni Dwi Putri reraeni@gmail.com Universitas Padjadjaran Master Study Program of Biomedical Sciences, Faculty of Medicine Bandung Indonesia - Dr. Norman T. Lubis Sulaiman Air Force Hospital Emergency Department Bandung Indonesia
Muhammad Iqbal Iskandar m.iqbal227@yahoo.com Dr. H. Abdul Moeloek Regional General Hospital Uro-Nephrology Department Bandar Lampung Indonesia -
Siska Juliana siskajuliana27497@gmail.com Dr. H. Abdul Moeloek Regional General Hospital Uro-Nephrology Department Bandar Lampung Indonesia -
Ahmad Faried ahmad.faried@unpad.ac.id Faculty of Medicine Universitas Padjadjaran - RS Unpad University Hospital Department of Neurosurgery and Stem Cell Working Group Jatinangor Indonesia -
Ria Bandiara ria.bandiara@unpad.ac.id Universitas Padjadjaran Faculty of Medicine Bandung Indonesia - Dr. Hasan Sadikin General Hospital Division of Nephrology and Hypertension, Department of Internal Medicine Bandung Indonesia
Rudi Supriyadi rudi.supriyadi@unpad.ac.id Universitas Padjadjaran Faculty of Medicine Bandung Indonesia - Dr. Hasan Sadikin General Hospital Division of Nephrology and Hypertension, Department of Internal Medicine Bandung Indonesia
 

Chronic kidney disease (CKD) heightens infection risk via immune dysregulation, repeated device exposure, and dialysis care. Although HD and CAPD pose access-related threats, dialysis patients also present with other diseases. In Indonesia, dialysis antibiograms are scarce, limiting empiric therapy and stewardship. We analyzed cultures and antimicrobial susceptibility testing (AST) from CKD patients on dialysis to map organism distribution by modality and specimen, quantify resistance to key agents, and identify clinical predictors of resistance to guide empiric choices.

Single-center retrospective cross-sectional study at Dr H Abdul Moeloek General Hospital Lampung (Jan 2024–Aug 2025). We include adults (≥18 y) with CKD on dialysis with positive cultures and AST; excluded no-culture records, non-clinically relevant contaminants, and duplicate isolates. The resistance rate was % resistant among isolates tested for an antibiotic. Logistic regression per antibiotic assessed associations between resistance (outcome) and age, gender, dialysis modality, specimen type, organism, and comorbidities; results as OR (95% CI), p-values.

We get 80 culture-positive isolate records from on dialysis CKD patients (71 HD, 9 CAPD). Specimens are accumulated from 13.75% CDL swab, 11.25% CAPD fluid , 23.75% pus, 16.25% blood, 25.00% sputum, 2.50% urine and 7.50% other body fluids. This shows infections come beyond access sites only. K. pneumoniae (15.0%), E. coli (13.75%), and S. aureus (13.75%) are the leading pathogens found from all isolates. In catheter-related infections, CDL samples showed S. epidermidis (27.27%, 95% CI 0.09–0.59) as most common organism, while K. pneumoniae (33.33%, 95% CI 0.11–0.67) and S. hominis (33.33%, 95% CI 0.11–0.67) were the main contributor in CAPD samples.

Fig 1. Distribution of Bacterial Isolates in CKD Patients undergoing Dialysis

Fig 2. Distribution of Bacterial Isolates Among HD and CAPD patients

Fig 3. Distribution of Bacterial Isolates by Specimen Type in CKD Patients Undergoing Dialysis

Resistance patterns vary by organism. We observed very high resistance to 3rd-generation cephalosporins and fluoroquinolones (≈90–100%) but remained fully susceptible to carbapenems and amikacin in E. coli. While K. pneumoniae shows moderate–high resistance to ceftriaxone (58.3%) and aztreonam (66.7%), but lower aminoglycoside resistance (16.7%). Half of S. aureus was MRSA (oxacillin/cefoxitin 50%) with vancomycin still susceptible and low clindamycin(9.1%) and erythromycin(18.2%) resistance.

Fig 4. Antibiotic Resistance Rates among Bacterial Isolates in CKD Patients Undergoing Dialysis

In exploratory modeling, organism type was the principal driver of resistance, though most organisms–antibiotic effects had wide confidence intervals. The most reliable association was lower tetracycline resistance in S. epidermidis (OR 0.012, 95% CI 0.0003–0.49; p=0.020). Dialysis modality and specimen type showed no independent association with resistance.

Infections in CKD dialysis patients come from both catheter related or non related sources. K. pneumoniae, E. coli, and S. aureus are most common organisms. The resistance landscape showed high β-lactam and fluoroquinolone resistance in Enterobacterales and persistent MRSA. Organism type showed significant relations to resistance rather than dialysis modality or specimen source. The importance of empirical therapy based on organisms are highlighted in these findings and the need for dialysis-related antibiograms to optimize outcomes in interconnected kidney care.

Kewords