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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Cisplatin is an effective but nephrotoxic agent used in oncology. Treatments such as hydration, bicarbonate therapy, and N-acetyl cysteine have not been successful in preventing nephrotoxicity.
Neutrophil elastase is a neutrophil-specific serine protease secreted from primary granules and plays an important role in inflammation. It has been shown that neutrophil elastase is overexpressed in conditions that cause acute kidney injury, such as sepsis and ARDS.
Sivelestat, a neutrophil elastase inhibitor, reduces elastase activation and inhibits neutrophil aggregation by decreasing the inflammatory response, IL-8 and tumor necrosis factor-α (TNF-α) concentrations.
This study evaluated the effect of sivelestat on preventing cisplatin-induced acute kidney injury.
In this experimental study, male and female Wistar rats weighing 270-320 g and aged 3 months were used.
The rats were divided into 5 groups, each containing 7 rats:
1. The first group was the control group, and this group received an intraperitoneal saline injection on the first day.
2. The second group was the cisplatin group (CP) and received 8 mg/kg cisplatin on the first day and IV saline intraperitoneally on the third day.
3. The third group was the sivelestat group (SIV) and received 50 mg/kg sivelestat on the first day and IV saline intraperitoneally on the third day.
4. The fourth group was the CP-SIVlow group and received 8 mg/kg cisplatin-50 mg/kg sivelestat on the first day and 50 mg/kg sivelestat intraperitoneally on the third day.
5. The fifth group was the CV-SIVhigh group and received 8 mg/kg cisplatin-100 mg/kg sivelestat on the first day and 100 mg/kg sivelestat intraperitoneally on the third day.
Sacrifice was performed on the fifth day. Blood samples were analyzed for urea, creatinine, phosphorus, and complete blood count. Proteinuria was evaluated in urine samples collected from rats kept in metabolic cages for 24 hours prior to sacrifice.
Histopathologically, vacuolar degeneration in tubular cells and Terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick end labeling (TUNEL) assay were evaluated by confocal microscopy.
Proteinuria was detected in the groups receiving cisplatin (groups 2, 4, and 5). The average laboratory results of the experimental animals are given in Table 1.
Table 1. Kidney function tests (mean)
urea
creatinin
phosphorus
Urea/cr
p
CONTROL
24,40
0,55
8,15
51,58
-
CP
48,82*
1,34*
10,90
60,08
<0,05*
SIV
18,20
0,45
5,60
49,38
CP+ SIV low
59,57*
0,90*
8,25
72,68
CP+ SIV high
49,67*
0,84*
7,47
62,60
The results of vacuolar degeneration evaluated with hematoxylin and eosin are shown in Table 2.
Table 2. Vacuolar degeneration results
0
(<5)
1
(%5-20)
2
(%20-50)
3 (>%50)
%3
%85
<0.05*
%2
%45
%40
TUNEL assay results were determined as 5%, 70% (p<0.05), 10%, 55%, and 50% for groups 1, 2, 3, 4, and 5, respectively.
In this study, sivelestat was shown to significantly improve renal function tests, reduce or reverse histopathological vacuolization, and decrease apoptosis in groups that developed cisplatin-induced nephrotoxicity. This study seems to be the first experimental study on this subject in the literature. However, future studies with larger sample sizes investigating this topic are needed.
