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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Obesity is a risk factor for hypertension, and it has been considered that volume overload is one of the mechanisms of blood pressure elevation in obesity. On the other hand, recent basic and clinical studies suggest that there is disequilibrium in water balance between the blood and each tissue. However, the effects of obesity on water balance at the tissue level are unclear. In this study, we examined the water balance between the blood and tissue levels in high-fat diet-induced obese mice. We also evaluated the association between Body Mass Index (BMI) and tissue water content in human skin.
Male 10-week-old C57BL/6J mice were fed a control (10% fat) or a high-fat diet (HFD, 45% or 60% fat) for 8 weeks. Circulating plasma volume was measured by Evans blue dye dilution. Relative water content in skin, skeletal muscle, kidneys, and liver was determined from the difference between wet and dry tissue weights. In a human study, we obtained skin samples from 51 consecutive patients who underwent laparoscopic rectal cancer surgery with colostomy. Relative skin water content was also measured, and its association with BMI was analyzed.
Compared to the control group, the HFD groups exhibited significantly higher blood pressure, body weight, and circulating plasma volume. On the other hand, while HFD did not alter the absolute water volume in each tissue, it significantly increased the dry weight due to lipid accumulation. Consequently, the relative water content (ml/g dry weight) in skin, skeletal muscle, and liver was significantly decreased. In a human study, consistent with the animal data, we found a significant negative correlation between BMI and skin water content in the human samples (R = -0.342, P = 0.014, N = 51, Spearman test).
These findings suggest that obesity leads to a paradoxical state of increased circulating plasma volume but a decrease in relative water content at the tissue level. This decoupling of water balance indicates that a state of "tissue-level dehydration" may exist despite plasma volume overload. Correcting this blood-tissue water imbalance could represent a novel therapeutic strategy for obesity-related pathologies.
