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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Kid Kidney regeneration is expected as an innovative therapy for patients with kidney failure. Under the concept of cellular interactions via conditioned media (CMs), we have attempted kidney repair by generating nephrons through direct injection of mesenchymal stromal cell (MSC)- or adipose-derived stem cell (ADSC)-differentiated tubular epithelial cells (TECs) into kidney cortices of rapidly progressive (RPKF) and chronic kidney failure (CKF) rats. Efficacy of our kidney cell injection therapy (K-CIT) was estimated by changes with time in serum creatinine (sCr) levels.
Hu Human MSCs and ADSCs were differentiated into TECs by cultivation for 4 weeks with collecting duct cell (CDC)-CM. Model rats of RPKF and CKF were each created by systemic administration of Adriamycin of 6.0 mg/kg BW and 3.5 mg/kg BW. These model rats (each n=5) were died within 12 weeks (A-12W) and 10 months after Adriamycin administration (A-10M). Differentiated TECs were pretreated with 3-dimensional (3-D) culture using a small amount of gel complex composed of Col-1 gel (60%/v), vascular endothelial cell (VEC)-CM (30%/v) and 10X Medium 199 (10%/v) for 2 weeks before implantation, which was the key step for successful nephron generation. And the TECs/gel mixture was implanted using 18-gauge injection needles into kidney cortices 2 weeks (A-2W) in 5 RPKF rats and 1 month (A-1M) in 5 CKF rats after Adriamycin administration. Blood samples for measuring sCr levels were collected from tail veins, and regenerated tissues were performed various IHC assays in addition to H&E, PAS and PAM stainings.
Serum Cr levels of MSC-derived TECs-implanted RPKF rats first showed significant difference (p<0.05) at I-7W, compared to those of the control rats. And the cell-implanted rats kept alive until euthanized A-14~18W, that is I-12~16W. Almost intact nephrons were regenerated at I-2W, and clearly damaged nephrons were scarcely observed until euthanized death. In CKF model, both MSC- and ADSC-derived TECs-implanted rats were alive with lower sCr levels now A-10~12M, that is I-9~11M. We will report the following course in addition to histological findings.
We could regenerate rich nephrons with the ability of ameliorating renal dysfunction in rat kidney cortices through K-CIT method with the implanted-cell pretreatment of 3-D culture using Col-1 gel and CDC-CM. It remains unclear how long our K-CIT method will be effective for KF patients, however, our method can be repeated against the same damaged kidney without ethical issues and immunological subjects. We hope that our K-CIT will spread over the world as a new therapeutic method for KF.
