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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Peritoneal dialysis (PD), a life-saving renal replacement therapy, is often complicated by peritoneal fibrosis, which limits long-term efficacy. Emerging evidence suggests that immune cells, particularly T cells, play a pivotal role in driving this fibrotic response. In this study, we explore the increased T cell infiltration and altered bioenergetics in the peritoneum of PD patients, shedding light on immune-metabolic pathways that may contribute to fibrosis and identifying potential therapeutic targets to improve PD outcomes.
Peritoneal biopsy tissue was obtained from ESRD patients on PD (n=15) undergoing catheter replacement or removal after renal transplantation. The peritoneal tissue for the control population (n=12) of the study was taken from persons with normal renal function during laparoscopic donor nephrectomy. T cell profiling and Bioenergtics studies using Single Cell ENergetIc metabolism by profiling Translation InHibition (SCENITH) by flow cytometry. Expression levels of key pro- and anti-fibrotic genes were measured by quantitative PCR (qPCR), and cytokine concentrations in culture supernatant were quantified using ELISA. Serum samples from patients and controls was used for invitro experments. Human renal fibroblast cell line TK 173 was used differentiation study.
Flow cytometric analysis revealed a significant increase in the frequencies of Th1, Th2, and Th17 cells, along with their corresponding functional markers, in PBMCs from peritoneal dialysis (PD) patients compared to healthy controls (P < 0.001). Cytokine profiling showed elevated levels of IFN-γ, IL-4, TGF-β, and IL-17 in patient samples. SCENITH analysis demonstrated that T cells from PD patients exhibited enhanced metabolic activity, with higher glycolytic (84%) and oxidative phosphorylation (OXPHOS, 27%) capacities, indicating a bioenergetic reprogramming that supports their increased proliferative and effector functions. This metabolic shift correlated with upregulated expression of key transcription factors (T-bet, GATA3, FOXP3, and RORγT; P < 0.001) and cytokines involved in T cell activation and differentiation (IFN-γ, IL-17, and IL-4; P < 0.0001). Gene expression analysis further confirmed elevated levels of these transcription factors in PBMCs from PD patients compared with controls (P < 0.0001). Moreover, when naïve CD4⁺ T cells isolated from healthy donors were cultured with serum from PD patients or controls, the patient serum induced a pronounced differentiation toward the Th2 phenotype (P < 0.001). Additionally, fibroblasts treated with patient serum showed significantly higher myofibroblast conversion and increased expression of fibrotic genes such as COL1A1, ACTA2 and FN1 (P < 0.0001) compared with those treated with control serum.
Targeting T cell metabolism may offer novel therapeutic avenues to mitigate fibrosis and improve the longevity of peritoneal dialysis.
