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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Fingerprint deposit (FPD) are observed by electron microscopy in lupus nephritis. However, the three-dimensional (3D) structure and composition of FPD remain unclear. Array tomography (AT) enables 3D-reconstruction of FPD using epoxy resion specimens. In this study, we performed 3D reconstruction of FPD using AT and component analysis using immunogold immunoelectron microscopy on existing epoxy resion specimens.
A sample was processed for AT and another for immunogold labeling. Serial ribbon sections were collected on conductive silicon wafers using AT and stained with 2% uranyl acetate and lead aspartate solutions. Serial images (1,188 µm area, 79 slices; 3,280 × 4,096 pixels × 70 nm thickness) were acquired using scanning electron microscope. Image alignment and segmentation and 3D reconstruction were performed by Fiji, Microscopy Image Browser, UNI-EM, and Amira software. To clarified IgG localization, ultrathin sections on nickel grids were treated with 0.25% trypsin in PBS at 37°C for 20 min, blocked with 1% BSA, and incubated overnight at 4°C with a 10 nm gold-conjugated anti-IgG antibody. After washing with Tris-buffered saline containing Tween-20, the sections were postfixed in 2% glutaraldehyde, stained with uranyl acetate, and examined by transmission electron microscope.
FDP were observed in the paramesangial, subendothelial and subepithelial electron dense deposits of lupus nephritis. The bands were approximately 10-17 nm thick, consistent with previous reports, and exhibited a fan-shaped, layered structure. Deep learning–based segmentation was effective for assessing the overall distribution but did not achieve the accuracy required for detailed 3D reconstruction. Therefore, manual layer-by-layer segmentation was performed to build the 3D model. The 3D structure of FPD was a regular layered structure resembling a mille-feuille.
Transmission electron microscopy detected 12–17 nm filamentous structures in the subendothelial deposits, suggesting the initiation of FPD formation. Then, several layers of bands are regularly arranged to form FPDs within the GBM. During the initial FPD passed through the glomerular basement membrane, layers of FPD were stratified, and mature FPD were observed in the epithelial area. Some subepithelial FDPs were endocytosed by podocytes, and were digested and excreted into the urinary lumen. Immunoelectron microscopy using 10-nm immunogold labeled for anti-IgG antibodies confirmed that the bands in FPD were composed of IgG.
The three-dimensional structure of FPD revealed that IgG was arranged in a regularly layered sheet-like structure resembling a mille-feuille. IgG filament structures are formed in the endothelium, which then form multi-layer structures through the glomerular basement membrane, forming mature FPD beneath the epithelium, which are then excreted into the urinary lumen.
