ESTABLISHING A METHOD TO PREDICT STEROID RESPONSIVENESS IN PEDIATRIC PATIENTS WITH IDIOPATHIC NEPHROTIC SYNDROME

Certificate Output Instructions

For best output, select "Paper Size" as "A4" and "Margin" as "0" or "None".

To save or print to PDF, please select Print Destination > Save as PDF, enable Background Graphics under "More Settings", then click "Save".

Presented the abstract " "
(Abstract co-author(s): )

Back

E-Poster Presentation

During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.

Preparing your E-Poster

Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.

E-Poster Submission Deadline

Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.

E-Poster Format Requirements

PDF file
Layout: Portrait (vertical orientation)
One page only (Dim A4: 210 x 297mm or PPT)
E-Poster can be prepared in PowerPoint (one (1) PowerPoint slide) but must be saved and submitted as PDF file.
File Size: Maximum file size is 2 Megabytes (2 MB)
No hyperlinks, animated images, animations, and slide transitions
Language: English
Include your abstract number
E-posters can include QR codes, tables and photos

E-Poster

https://storage.unitedwebnetwork.com/files/1099/73037485a7f8524327bcda384df9b955.pdf

Abstract Title *

ESTABLISHING A METHOD TO PREDICT STEROID RESPONSIVENESS IN PEDIATRIC PATIENTS WITH IDIOPATHIC NEPHROTIC SYNDROME

Please follow the instructions below to input your abstract title.

Abstract titles should be brief and reflect the content of the abstract.

The title will not be accepted if it exceeds 25 words.
Type in CAPITAL LETTERS.
Lowercase may be used for abbreviations only, for example, mRNA.

Co-author 1

Chikage Yajima chikageyo@gunma-u.ac.jp Gunma University Graduate School of Medicine Pediatrics Maebashi Japan *

Co-author 2

Yasuko Kobayashi kobayasu@gunma-u.ac.jp Gunma University Graduate School of Medicine Pediatrics Maebashi Japan -

Co-author 3

Yoko Yamasaki y.takagi@gunma-u.ac.jp Gunma University Graduate School of Medicine Pediatrics Maebashi Japan -

Co-author 4

Co-author 5

Co-author 6

Co-author 7

Co-author 8

Co-author 9

Co-author 10

Co-author 11

Co-author 12

Co-author 13

Co-author 14

Co-author 15

Introduction

Steroid responsiveness is important to forecast long-term renal prognosis in patients with idiopathic nephrotic syndrome. However, there is no biomarker/method to predict steroid responsiveness at disease onset. Patients must undergo > 4 weeks of steroid treatment before being determined steroid resistant. These patients may experience severe adverse effects as well as complications of refractory edema. Therefore, establishing methods for predicting steroid responsiveness at disease onset is essential. We aimed to establish a method to predict steroid responsiveness in pediatric patients with idiopathic nephrotic syndrome.

Methods

We investigated αvβ3 integrin activation, cell migration, and active Rho GTPases in healthy human podocyte cell lines (ciPods). The cells were stimulated with patients’ serum obtained from steroid sensitive and steroid resistant during protein uric periods. Activated αvβ3integrin expressed on the basement membrane of the podocytes was measured using an immunofluorescent antibody method with total internal reflection fluorescence (TIRF) microscopy. Cell migration activity was evaluated by scratch assay. Active GTPases were measured by ELISA.

Results

Steroid resistant serum (n=9) activated αvβ3 integrin to a significantly greater extent (P=0.0012) than did steroid sensitive serum (n=14).This test had 78% sensitivity, 93% specificity and positive predictive value of 88% with 1.792 cut off point. Cell migration occurred significantly faster with steroid resistant serum (n=5) than with steroid sensitive serum (n=5) (P=0.0009 at 18 h, and P=<0.0001 at 24 h).Small GTPase activation assays showed small tendency for increased Cdc42 activation and decreased RhoA activation following treatment with steroid resistant serum (n=6) compared with steroid sensitive serum (n=6).

Conclusion

αvβ3integrin activation assay using healthy human ciPods imaged with TIRF microscopy may be useful in distinguishing pediatric patients with steroid-resistant nephrotic syndrome from those with steroid-sensitive. The migration assay results functionally support the effect of αvβ3integrin activation on morphofunctional modifications in podocytes. Further investigation with increased sample sizes at disease onset is required. The content presented in this abstract was submitted for other meetings.(American Society of Nephrology Kidney Week 2024)

Kewords