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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Chronic kidney disease (CKD) is closely linked to increased cardiovascular morbidity and mortality, with the uremic toxin indoxyl sulfate (IS) identified as a key contributor to disease progression. IS promotes vascular inflammation and accelerates atherosclerosis. In a previous study, we found that granola consumption may reduce serum IS levels in hemodialysis patients. Avenanthramide C (Ave), a polyphenol derived from oats, has antioxidant and anti-inflammatory properties; however, the precise mechanism underlying its anti-inflammatory effect remains unclear. This study aimed to determine whether Ave could suppress IS-induced inflammatory responses in endothelial cells and to elucidate the underlying molecular mechanisms.
Human umbilical vein endothelial cells (HUVECs) were cultured under the following conditions: vehicle control, IS (50 µg/mL), and IS co-treated with Ave (10 µM). After 24 hours, both culture supernatants and cells were collected for analysis. The following experiments were conducted: Total RNA was extracted and subjected to RNA sequencing (RNA-seq). To examine the involvement of specific signaling pathways, α-adrenergic receptor blockers, AMPK inhibitors, and Akt inhibitors were applied, and interleukin-6 (IL-6) levels were measured by ELISA. Molecular docking simulations were used to analyze the binding interactions between IS or Ave and the aryl hydrocarbon receptor (AhR). Nuclear translocation of NF-κB was assessed via western blotting. Expression of the AhR target gene Cyp1a1 was measured by quantitative PCR. Intracellular uptake of Ave was evaluated using high-performance liquid chromatography (HPLC).
RNA-seq results indicated that Ave may exert anti-inflammatory effects by modulating the PI3K/Akt/IκB pathway and inhibiting nuclear translocation of NF-κB. The anti-inflammatory effect of Ave was not diminished by α-adrenergic receptor blockers, AMPK inhibitors, or Akt inhibitors, suggesting that these pathways are not essential to its mechanism of action. Docking simulations suggested that Ave may act as a competitive inhibitor of AhR. Ave significantly suppressed IS-induced nuclear translocation of NF-κB and the upregulation of Cyp1a1. Intracellular presence of Ave was confirmed 24 hours after treatment, indicating successful uptake into HUVECs.
These findings suggest that Ave is taken up by endothelial cells, functions as a competitive antagonist of AhR, and attenuates IS-induced inflammation via the AhR/NF-κB pathway. Therefore, granola consumption may not only reduce serum IS levels but also inhibit IS-mediated inflammation, thus contributing to protect against developing cardiovascular disease in hemodialysis patients.
