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Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Arteriosclerosis-related vascular disease is well known to impair quality of life and significantly impact on life prognosis. In hemodialysis patients, arteriosclerosis-based vascular disorders can lead to limb amputation or infection, which can be fatal. Therefore, early diagnosis and the establishment of treatments are urgently issues.
The Ankle-Brachial Index and Toe-Brachial Index (ABI/TBI) are widely used in routine clinical practice to diagnose arteriosclerosis due to their ease of administration.
We retrospectively analyzed 102 hemodialysis patients who were followed over a 10-year period from 2007 to 2016 to examine the relationship between ABI/TBI and cardiovascular and cerebrovascular deaths and to assess its efficacy for early diagnosis.
In addition, we separately examined relationship between changes in the triglyceride to cholesterol ratio in lipoproteins and oxidative stress.
1. Long-Term Observation Study
The subjects were 102 hemodialysis patients (52 women, 50 men), with an initial age of 58 ± 13years. Of these, 34 patients were free of arteriosclerotic complications (Group S; age 50±12 years, dialysis duration 134±95 months), 43 patients were found to have arteriosclerotic complications (Group A; age 61±9 years, dialysis duration 109±72 months), and 25 patients died from cardiovascular or cerebrovascular disorders (Group D; age74±7 years, dialysis duration 114±61 months).
ABI/TBI Measurement
Measurements were conducted using a Va Sera VS-3000 (Fukuda Denshi, Tokyo, Japan) after 5 minutes of bed rest in a controlled environment at a room temperature of 25°C and humidity of approximately 60%.
Serum Lipid Analysis
Blood was collected at the beginning of the week before the start of hemodialysis, and serum was stored in frozen state. After serum electrophoresis using an electrophoresis device (Heparizer, Helena Laboratory, Omiya, Japan), cholesterol and triglycerides were double-stained and quantified by densitometry, and their ratios in lipoproteins were calculated.
2. Study on Oxidative Stress
Nineteen healthy adults (16 women, 3 men, age 51± 12 years) and 22 maintenance hemodialysis patients (16 women, 6 men, age 75 ± 7 years, duration of dialysis 171 ± 133 months) were analyzed. Lipid analysis was performed in the same manner as above. The serum reactive oxygen species scavenging activity of 6 radicals, namely superoxide, singlet oxygen, alkoxyl radical, peroxyl radical, hydroxyl radical, and methyl radical, was measured
using electron spin resonance device (FR80, JEOL Co., Ltd. Japan).
Study 1.
The ABI and TBI for each group in the first and tenth years are as follows: ABI, S:1.22±0.08→1.10±0.13, A: 1.22±0.11→1.01±0.14, D: 1.10±0.25→0.88±0.07, TBI, S: 0.90±0.17→0.74±0.14, A: 0.84±0.16→0.64±0.14, D: 0.67±0.17→0.49±0.09 respectively.
The ABI of patients who died of vascular disorders was within the normal range, but TBI was below 0.7 from the start of testing and showed declining over time. In surviving patients, both ABI and TBI decreased over time, but both remained within normal limits. Among surviving patients with non-fatal cardiovascular complications or peripheral arteriosclerosis, only TBI decreased significantly over time.
The triglyceride ratio in LDL was significantly higher in the HD patient group. S: 0.27±0.06 →0.29±0.08, A: 0.29±0.06→0.32±0.06, D: 0.26±0.05→0.34±0.02, respectively. A significant inverse correlation was observed between TBI and triglyceride ratio.
Study 2.
Serum ROS scavenging activity in healthy subjects and HD patients was imbalanced: methyl radical (GSH mM equivalents) 161±124 and 34±19, superoxide (SOD Unit equivalents) 0.20±0.07 and 0.58±0.14, and alkoxyl (Trorox mM equivalents) 1.35±0.33 and 1.80±0.35. The LDL Tg ratio was 0.22±0.03 and 0.31±0.06, respectively, showing a significant increase in HD patients. TBI was 0.85±0.15 and 0.59±0.19, respectively.
Furthermore, the results of ROS scavenging activity revealed that oxidative stress alters lipid profiles and contributes to the progression of arteriosclerosis.
These results suggest that TBI can be used to assess arteriosclerosis earlier than ABI and can aid in determining therapeutic intervention.
