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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Mice are widely used in genetic research due to their diverse genetic backgrounds, including inbred and mutant strains. Their small size, ease of breeding, and short generation time make them ideal for genetic analysis. Advances in DNA polymorphic markers such as microsatellites and YAC libraries have facilitated linkage and physical mapping. The vast data accumulated is now searchable online, further increasing the utility of mice. In contrast, rat models are predominantly used in kidney disease research, with limited application of mice. One reason is the difficulty in collecting sufficient urine. Metabolic cages yield only ~100 μL of urine, which may evaporate under high temperatures. Forced hydration via gastric intubation is non-ethical and may induce stress-related bias, with minimal increase in water intake. To address this, we developed a method to enhance voluntary water consumption by flavoring drinking water, aiming to improve urine collection.
Six strains of 8-week-old mice (B6, Balb/c, B10, B10A, B10-BR, B10D2) were placed in metabolic cages for 24-hour urine collection. Body weight, blood pressure, and blood glucose were measured before and after urine collection. Urine volume, water intake, and urine dipstick findings were recorded. Drinking water was flavored with sports drinks (Pocari Sweat, Otsuka Beverage), coconut milk, and the sweetener stevia, and compared with pure water.
Coconut milk did not increase urine volume. Undiluted sports drinks yielded ~10 mL of urine over 24 hours but caused marked hyperglycemia. Both sports drinks and stevia showed reduced urine volume with higher dilution. When Pocari Sweat and stevia were administered at 6-fold dilution or ≥0.01% concentration, no changes were observed in blood glucose, blood pressure, or renal function. Strain-dependent differences in water intake and urine volume were noted. Notably, Balb/c mice showed no increase in water intake or urine volume in response to taste stimulation. The T1R family of G protein-coupled receptors (GPCRs) mediates umami and sweet taste perception. The T1R1/T1R3 heterodimer detects umami, while the T1R2/T1R3 heterodimer detects sweetness. Polymorphisms in T1R3 affect saccharin sensitivity across mouse strains; Balb/c mice are saccharin-insensitive, likely explaining their weak response to sweet stimuli. Additionally, B10 congenic strains show differential responses to toluene exposure, possibly due to differences in MHC haplotypes.
Taste stimulation can safely increase water intake and yield sufficient urine for downstream studies without affecting blood glucose, blood pressure, or renal function. I declare that this abstract was also submitted for the 65th. Annual Meeting of Japanese Society for Nephrology and I also declare that re-submitting the abstract is permitted by the organizers of the original meeting.
