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During the congress, E-Posters will be accessible to all participants on the congress website 24/7, as well as in the E-poster stations in the congress center.
Preparing your E-Poster
Please review the E-Poster format requirements carefully when preparing your E-Poster. Should your E-Poster not meet the mentioned requirements, it may not be displayed as described above.
E-Poster Submission Deadline
Please prepare and upload your E-Poster no later than March 14, 2026 11.59PM CET. After this date, you will no longer be able to prepare and upload your E-poster and it will not be displayed and accessible on the congress website.
Please follow the instructions below to input your abstract title.
Abstract titles should be brief and reflect the content of the abstract.
Recent advances in stem cell biology have enabled the generation of artificial kidney tissues (kidney organoids) from pluripotent stem cells (PSCs). Recently, the successful generation of kidney organoids with complex three-dimensional structures (higher-order structures) from mouse embryonic stem cells (ESCs) has been reported. However, these kidney organoids are missing the ureter, the excretory pathway for the produced urine. This presents a significant challenge in the application of kidney organoids in transplantation medicine.
The ureter is composed of the epithelium and surrounding stroma, and it develops through the interaction of these progenitors. While several induction protocols for ureteral epithelial progenitors (Ureteric Buds) from PSCs have been reported, no method has been established worldwide for inducing the remaining ureteral stromal progenitors. Furthermore, there have been no reports of successful generation of artificial ureteral tissues (ureteral organoids) from PSCs.
The present study investigated the induction conditions for ureteral stromal progenitors from PSCs. Furthermore, a method for integrating the induced ureteral stromal progenitors with ureteral epithelial progenitors has been developed. The utilization of these methods was undertaken to facilitate the generation of ureteral organoids from PSCs.
Initially, we utilized mouse embryonic kidneys and ureters to identify specific genes of ureteral stromal progenitors and their developmental mechanisms. Subsequently, we isolated the tissues of posterior intermediate mesoderm, the origin of ureteral stromal progenitors, from mouse embryos and determined the culture conditions for inducing them into ureteral stromal progenitors. In light of the aforementioned findings, we established induction protocols for ureteral stromal progenitors from mouse ESCs and human induced pluripotent stem cells (iPSCs) via the posterior intermediate mesoderm. Additionally, a reconstitution method was developed for combining these two progenitors in vitro. The utilization of these methods resulted in the successful generation of differentiated ureteral organoids that exhibited peristaltic constructions, achieved through the integration of ureteral stromal progenitors derived from PSCs with ureteral epithelial progenitors derived from mouse embryos or induced from PSCs. Furthermore, these induction protocols for ureteral stromal progenitors and reconstitution assay system enabled the modeling of pathological states affecting the ureter.
We have revealed the developmental program for ureteral stromal progenitors and successfully induced them from mouse and human PSCs in vitro. The reconstitution of induced ureteral stromal progenitors and ureteral epithelial progenitors, derived from mouse embryos or induced from PSCs, has enabled the generation of ureteral organoids. Furthermore, we demonstrated the potential of ureteral organoids as a disease model for ureter-affecting conditions. The present study offers a valuable foundation for elucidating the pathological and developmental mechanisms of the ureter, thereby facilitating progress towards the development of transplantable kidney organoids that exhibit urine flow.
