C3 GLOMERULONEPHRITIS AND THROMBOTIC MICROANGIOPATHY - A PROSPECTIVE OBSERVATIONAL STUDY

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C3 GLOMERULONEPHRITIS AND THROMBOTIC MICROANGIOPATHY - A PROSPECTIVE OBSERVATIONAL STUDY
Mythri
Shankar
Ranjitha Siddalingappa ranjithasraate@gmail.com Institute of Nephrourology Nephrology Bengaluru
Sreedhara C Gurusiddaiah ranjithasraate1@gmail.com Institute of Nephrourology Nephrology Bengaluru
Kishan Aralapuram s_mythri@yahoo.com Institute of Nephrourology Nephrology Bengaluru
Girish Reddy kisan@gmail.com Institute of Nephrourology Nephrology Bengaluru
Karthik Tennankore ktennankore@gmail.com University of Dalhousie Nephrology Dalhousie
 
 
 
 
 
 
 
 
 
 

Complement mediated TMA (cTMA) and complement component 3 glomerulonephritis (C3GN) are two distinct complement mediated kidney diseases. cTMA is characterized by acute kidney injury, thrombocytopenia and microangiopathic hemolytic anemia. C3GN results from uncontrolled activation of the alternative complement pathway leading to the deposition of C3 in the glomerulus. We sought to describe characteristic differences between these two conditions in a large cohort of patients from Bengaluru, India. 

All cases of cTMA and kidney biopsy-proven C3GN from 2019-2022 at a tertiary care center from South India were included. Patients with C3GN had persistently low C3 for more than 12 weeks and no identifiable source of infection. All patients received protocolized medical therapy; patients with C3GN were treated with steroids and cyclophosphamide, while those with complement mediated TMA received plasmapheresis in addition to cyclophosphamide and steroids. Protocolised medical management (renin-angiotensin-aldosterone inhibition and diuretics) was provided in both the cases.  Demographic, clinical and lab parameters were reported  and compared.

A total of 25 cTMA cases and 19 C3GN cases were included in the study. Both C3GN (52.7%) and cTMA (48%) were more common in males. Patients with C3GN typically presented in the fourth decade of life while cTMA cases presented in the third decade. Secondary hypertension was common in the cTMA group (92% vs 78.9%, compared to C3GN). 60% of patients with cTMA had either recent or current infection compared to 21.1% of C3GN patients (P=0.04). The majority of cTMA cases had IFTA of <25% (84%)  compared to C3GN (57.9%) although this was not statistically significant. Both C3GN and cTMA most commonly presented as rapidly progressive kidney failure and acute kidney injury. A total of 76% of patients in the cTMA group required kidney replacement therapy as compared to 47.4% in the C3GN group (P=0.009). 44% of cTMA cases had anti-factor H antibody positivity compared to 15.8% of C3GN patients (P=0.02). Finally, 44% of cases in cTMA group had complete recovery compared to 47.4% in C3GN group at the end of 2.8 years of follow-up


VARIABLES

Diagnosis

P value

cTMA

C3GN

Age in years

26.72±19.26

31.37±18.84

0.200

Serum Creatinine (mg/dl)

7.04±4.28

6.18±5.80

0.104

Proteinuria (g/day)

1.87±1.91

2.84±2.90

0.001**

Haemoglobin (g/dl)

8.49±2.21

8.53±1.54

0.143

Total White Cell Count

10952.00±6062.94

8760.53±3696.55

0.343

Platelet Count

160996.08±120986.46

255336.84±179247.81

0.034*

Sodium (mEq/l)

135.04±4.76

136.42±3.66

0.168

Potassium (mEq/l)

4.26±0.80

4.44±0.62

0.845

Urea

107.24±43.90

99.96±70.35

0.200

Serum Albumin

3.43±0.67

3.02±0.77

0.124

Total Follow up

2.88±0.88

2.79±0.92

0.149



Recovery

Diagnosis

P Value

TMA

C3GN

C3GN

VS TMA

COMPLETE RECOVERY

11(44%)

9(47.4%)

0.677

PARTIAL RECOVERY

7(28%)

3(15.8%)

NO RECOVERY

7(28%)

7(36.8%)

Total

25(100%)

19(100%)

 

Secondary HTN, association with infection, anti-factor H positivity and requirement of KRT were predominant in the cTMA group compared to C3GN group. Though both the diseases are due to alternate pathway dysregulation, the clinical features and prognosis are variable, with cTMA

having a poorer prognosis compared to C3GN.



 Light microscopy (H&E):Thrombotic microangiopathy

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