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Renal injury is a main complication in cholestasis. Conventional biomarkers for kidney function such as plasma urea levels (Up) and glomerular filtration rate (GFR) have a diagnostic value when renal function has already lost. Our group has worked in the search for urinary biomarkers that could early indicate the presence of renal dysfunction in other pathologies.
The aim of this work was to evaluate the urinary excretion of renal tubular proteins, Caveolin-1 (Cav-1u) and Caveolin-2 (Cav-2u) as potential biomarkers of early renal damage induced by obstructive cholestasis in male Wistar rats.
Obstructive cholestasis was induced by Bile Duct Ligation (BDL) for 21h (BDL21h, n=5) and 72h (BDL72h, n=6). Sham-operated control group (Sham, n=7) was also processed (Institutional Animal Care and Use Committee, FBIOyF-UNR, Res. 657/2016). Up was determined; GFR was calculated by creatinine clearance (CrCl). The urinary excretion of Neutrophil Gelatinase-Associated Lipocalin (NGALu), a urinary biomarker of kidney injury, Cav-1u and Cav-2u were evaluated by immunoblotting. ANOVA-Newman Keuls, P<0,05: (a) vs. Sham, (b) vs. BDL21h, (c) vs. BDL72h.
(Mean±SEM) Up(g/L) Sham=0.44±0.03, BDL21h=0.41±0.02, BDL72h=0.45±0.04; CrCl (mL/min.100g): Sham=0.44±0.02, BDL21h=0.47±0.02, BDL72h=0.35±0.01a,b; Cav-1u(%): Sham=100±2, BDL21h=145±14a, BDL72h=145±8a; Cav-2u(%): Sham=100±3 , BDL21h=138±8a, BDL72h=133±8a; NGALu(%): Sham=100±3, BDL21h=118±8, BDL72h=124±9a.
Up was not altered while CrCl and NGALu were significant increased after 72h of BDL. Cav-1u and Cav-2u were significant increased at 21h after BDL.
These results provide evidence for Cav-1u and Cav-2u as potential early biomarkers of renal damage induced by obstructive jaundice. This could be a strategy to predict cholestasis-induced renal injury.
This abstract was also submitted for the PANAM Physiological Sciences 2023 congress. I also declare that re-submitting the abstract is permitted by the organizers of the previous meeting.