The role of Ginsenoside Rg3 in Ischemia Reperfusion renal Injury Mice via AMPK and autophagy.

Back

E-Poster

Abstract Title

First Name *

Kyungho

Last Name *

Park

Co-author 1

Dae Eun CHOI daenii@cnu.ac.kr Chungnam National University Medical Science Daejeon

Co-author 2

Ki Ryang Na drngr@cnu.ac.kr Chungnam National University Hospital Nephrology Daejeon

Co-author 3

Young Rok Ham youngrok01@cnuh.co.kr Chungnam National University Hospital Nephrology Daejeon

Co-author 4

Eu Jin Lee eujinlee@cnuh.co.kr Chungnam National University Hospital Nephrology Daejeon

Co-author 5

Hyerim Park Park hye05240@gmail.com Chungnam National University Medical Science Daejeon

Co-author 6

Yoon-Kyung Chang racer@catholic.ac.kr Daejeon Saint Mary Hospital Nephrology Daejeon

Co-author 7

Co-author 8

Co-author 9

Co-author 10

Co-author 11

Co-author 12

Co-author 13

Co-author 14

Co-author 15

Introduction

The benefitial role of Ginsenoside Rg3 (Rg3) have been demonstrated through different mechanisms. However, the specific evaluation of its renal-protective effect and the involvement of autophagy remain unclear. This study aims to examine how Rg3 induces autophagy flux and diminishes renal cell death in renal ischemia reperfusion injury (IRI).

Methods

C57Bl/6 mice were categorized into the subsequent groups: sham; sham treated with Rg3; IRI mice treated with saline; IRI mice treated with Rg3. Kidneys and blood samples were obtained 24 hours after the surgical procedure (sham and IR operation). Renal function, kidney histology, and the protein expression of autophagy markers were assessed.

Results

In the IRI mice group, there was an elevation of BUN and s-Cr levels compared to the sham group. However, the administration of Rg3 resulted in a reduction of BUN and s-Cr levels in the IRI mice. Furthermore, Rg3 treatment led to a decrease in renal injury, as evidenced by a lower renal tubular cell detachment and necrosis score in the IRI mice. The Rg3-treated IRI mice exhibited reduced oxidative stress and improved autophagy, characterized by increased levels of LC3 and Beclin-1, decreased levels of p62, and higher levels of renal ATP6E compared to the IRI mice treated with saline. Additionally, Rg3 treatment promoted the phosphorylation of AMPK in the kidneys of IRI mice.

Conclusions

Ginsenoside Rg3 (Rg3) induces activation AMPK-induced-autophagy flux and diminishes renal cell death in acute renal renal injury via ischemia reperfusion.

E-Poster Format Requirements

PDF file
Layout: Portrait (vertical orientation)
One page only (Dim A4: 210 x 297mm or PPT)
E-Poster can be prepared in PowerPoint (one (1) PowerPoint slide) but must be saved and submitted as PDF file.
File Size: Maximum file size is 2 Megabytes (2 MB)
No hyperlinks, animated images, animations, and slide transitions
Language: English
Include your abstract number
E-posters can include QR codes, tables and photos