APOL1 RISK VARIANTS AND CHRONIC KIDNEY DISEASE (CKD) IN RURAL UGANDA AND KENYA: THE SEARCH-CKD STUDY

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E-Poster

https://storage.unitedwebnetwork.com/files/1099/2c8614ea950f7bdb93ec350066c93100.pdf

Abstract Title

First Name *

Anthony

Last Name *

Muiru

Co-author 1

James Ayieko jimayieko@gmail.com Kenya Medical Research Institute Center for Microbiology Research Kisumu

Co-author 2

Jane Kabami kabamijane@gmail.com Infectious Diseases Research Collaboration Infectious Diseases Research Collaboration Kampala

Co-author 3

Mucunguzi Atukunda mucungua@gmail.com Infectious Diseases Research Collaboration, Infectious Diseases Research Collaboration, Kampala

Co-author 4

Gordon Orori ororigordon@gmail.com Kenya Medical Research Institute Center for Microbiology Research Kisumu

Co-author 5

Mona Abraham mabrah16@jhmi.edu Johns Hopkins University School of Medicine Medicine Baltimore

Co-author 6

Wendy Chan Wendy.Chan@ucsf.edu University of California, San Francisco Departments of Pediatrics, San Francisco

Co-author 7

Matthew Hickey Matt.Hickey@ucsf.edu University of California, Medicine San Francisco

Co-author 8

Edwin Charlebois Edwin.Charlebois@ucsf.edu University of California, Medicine San Francisco

Co-author 9

Moses Kamya mkamya@idrc-uganda.org Makerere University College of Health Sciences Kampala

Co-author 10

Maya Petersen mayaliv@berkeley.edu University of California, Berkeley School of Public Health Berkeley

Co-author 11

Diane Havlir Diane.Havlir@ucsf.edu University of California, Medicine San Francisco

Co-author 12

Michelle Estrella Michelle.Estrella@ucsf.edu University of California, Medicine San Francisco

Co-author 13

Chi-yuan Hsu Chi-yuan.Hsu@ucsf.edu University of California, Medicine San Francisco

Co-author 14

Co-author 15

Introduction

Apolipoprotein L1 (APOL1) high-risk genotypes (G1/G1, G1/G2 or G2/G2) are associated with an increased risk of kidney disease among people of recent sub-Saharan Africa ancestry. However, there are few large-scale population-based studies which have quantified genotype frequency in East Africa.

Methods

Sustainable East Africa Research in Community Health (SEARCH, NCT01864603) was a community cluster-randomized HIV test and treat trial. Within each study community, we selected 100 households with at least one HIV-positive adult and 100 households without any HIV-positive adults. Subsequently, we randomly chose one HIV-positive and one HIV-negative adult from the respective households to participate in the SEARCH-CKD study (PMID 32130245). After obtaining informed consent, we collected dried blood spots (DBS) on filter cards to genotype for APOL1 risk variants which were defined using custom designed TaqMan® SNP genotyping assays (rs73885319 and rs60910145 for the G1 haplotype, and rs71785313 for the G2 haplotype). We used multivariable logistic regression models to assess the association of APOL1 high-risk genotypes (G1/G1, G1/G2 or G2/G2) with prevalent CKD defined as a serum creatinine-based eGFR <60 mL/min/1.73m2 or proteinuria (urine dipstick ≥1+).

Results

Conclusions

We identified a relatively low prevalence of APOL1 risk variants in rural Uganda and Kenya—which strengthens the literature regarding the distinctive West-East Africa cline (high to low) in APOL1 risk allele distribution previously reported. Given the low frequency of APOL1 high-risk genotypes in East Africa, this does not appear to be a major driver of the burden of CKD in this region.

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