Antioxidant effects of a mix containing Curcuma longa L. and green propolis extract on patients undergoing hemodialysis: results from a randomised clinical trial

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Antioxidant effects of a mix containing Curcuma longa L. and green propolis extract on patients undergoing hemodialysis: results from a randomised clinical trial
Livia
Alvarenga
Danielle Nascimento danidesouzan@gmail.com Federal University of Rio de Janeiro Graduate Program in Biological Sciences – Physiology Rio de Janeiro
Heloiza Couto heloizanutri@hotmail.com Fluminense Federal University Graduate Program in Nutrition Sciences Niterói
Isadora Britto isadorabkopke@gmail.com Federal University of Rio de Janeiro Graduate Program in Biological Sciences – Physiology Rio de Janeiro
Livia Alvarenga liviaalvarenga92@gmail.com Federal University of Rio de Janeiro Graduate Program in Biological Sciences – Physiology Rio de Janeiro
Ludmila Cardozo ludmila.cardozo@gmail.com Fluminense Federal University Graduate Program in Nutrition Sciences Niterói
Andresa A. Berretta andresa.berretta@apisflora.com.br Apis Flora Indl. Coml. Ltda Research, Development & Innovation Department Ribeirão Preto
Marcelo Ribeiro-Alves mribalves@gmail.com National Institute of Infectious Diseases Evandro Chagas (INI/Fiocruz) HIV/AIDS Clinical Research Center Rio de Janeiro
Virgílio Pimentel Delgado virgiliodelgado@gmail.com Renal Medical Assistance Renal Medical Assistance Teresópolis
Danielle Figueiredo da Cunha daniellemedicina@hotmail.com Renal Medical Assistance Renal Medical Assistance Teresópolis
Anderson J. Teodoro andersonteodoro@id.uff.br Fluminense Federal University Graduate Program in Nutrition Sciences Niterói
Carolina Almeida carolina.almeida@edu.unirio.br Federal University of the State of Rio de Janeiro Graduate Program in Food and Nutrition Rio de Janeiro
Isabela Lemos isabelalemos10@gmail.com Fluminense Federal University Graduate Program in Nutrition Sciences Niterói
Mônica Martins Guimarães Guerra monicaguerra@unifeso.edu.br Renal Medical Assistance Renal Medical Assistance Teresópolis
Denise Mafra dmafra30@gmail.com Federal University of Rio de Janeiro Graduate Program in Biological Sciences – Physiology Rio de Janeiro
 

Oxidative stress is a common complication in chronic kidney disease (CKD) patients, increasing cardiovascular disease risk. Nutritional strategies are being proposed, including using bioactive compounds in foods to mitigate oxidative stress since these compounds can be a scavenger of reactive oxygen species (ROS). Turmeric and propolis are foods that have antioxidant and anti-inflammatory capacity. However, no studies have shown the effects of these foods, especially as a mix, on the redox balance in patients with CKD. The study's aim is to evaluate the effects of propolis and turmeric supplementation on redox balance in patients with CKD undergoing hemodialysis (HD).

Double-blind, randomised clinical trial with patients with CKD undergoing HD who received 10 mL of a mix containing a 110 mg/day standardised extract of green propolis (EPP-AF) associated with turmeric extract (with 130 mg of curcuminoids/day) or placebo for 8 weeks. The analysis of antioxidant capacity was performed by the oxygen radical absorption capacity test (ORAC), iron reduction power test (FRAP), and the test of 2,2-diphenyl-1-picrilhydrazil (DPPH). The determination of malondialdehyde was estimated by lipid peroxidation. 

The study involved 35 patients: 17 in the intervention group (51 ± 19 years; 12 males; 45.5 ± 25 months in HD; BMI= 23.7± 5.4 kg/m2) and 18 in the placebo group (51.5 ± 12.2 years; 9 males; 57.5 ± 54 months in HD; BMI= 23.4 ± 3.8 kg/m2). The data revealed that the intervention with propolis and turmeric increased antioxidant capacity (figure 1), showing a significant difference in the FRAP test (p-value= 0.0178). There was also a significant response in ORAC and DPPH tests in both groups. MDA plasma levels did not change after intervention in both groups.

Conclusions

The mix containing turmeric and propolis offered for 8 weeks improved the antioxidant response of hemodialysis patients

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