Introduction:
Processing bodies (P-bodies) are conserved cytoplasmic structures involved in mRNA turnover, playing key roles in mRNA decay and silencing. While some mRNAs are degraded in P-bodies, others are protected and can re-initiate translation. In podocytes expressing miR193a, the formation of P-bodies and their role in mRNA regulation remain under study. miR193a podocytes show increased pro-apoptotic factors and degradation of podocyte-specific proteins like WT1 and CD2AP, potentially contributing to podocyte dysfunction. Here in this study, we evaluated the formation of P-bodies in podocytes with induced miR193a expression.
Methods:
Stable human PD cell lines expressing miR193a were developed. PDs were differentiated (DPDs) and evaluated for composition of P-body formation. The formation of P Bodies was analysed by confocal microscopy followed by western blotting. To determine the role of miR193a on the stability WT1 and CD2AP, DPDs were transfected and were evaluated for the altered podocyte protein expression through confocal microscopy, real time PCR and western blotting
Results:
miR193a induced expression has decreases the P bodies accumulation in DPD. An increased expression of the hDcp1a protein indicated towards lower stability of podocyte specific proteins through mRNA decapping. miR193a expression found to be inversely correlated with LSM14 while in direct regulator of hEDC4, hDcp1a, and hDcp2a
Conclusions:
Our study shows that miR193a expression in podocytes decreases P-body formation, as evidenced by higher hDcp1a protein levels, which suggests enhanced mRNA decapping and destabilization of key podocyte proteins like WT1 and CD2AP. miR193a also downregulates LSM14 while upregulating hEDC4, hDcp1a, and hDcp2a, further supporting its role in promoting mRNA decay and podocyte dysfunction.
I have no potential conflict of interest to disclose.
I did not use generative AI and AI-assisted technologies in the writing process.